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In 1979, the city became a UNESCO World Heritage Site. The town's 2300 years of continuous urban tradition (Greek, Roman, Venetian, Habsburg) have left a fascinating and unique concentration of palaces, churches, towers, and fortifications.In 1997, Trogir was inscribed onto the UNESCO list of World Heritage Sites.We applied zooarchaeology by mass spectrometry (Zoo MS) to find additional hominin remains.

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The data show that all the Neanderthal remains are from a much earlier period (Previous dating of the Vi-207 and Vi-208 Neanderthal remains from Vindija Cave (Croatia) led to the suggestion that Neanderthals survived there as recently as 28,000–29,000 B. Subsequent dating yielded older dates, interpreted as ages of at least ∼32,500 B. We have redated these same specimens using an approach based on the extraction of the amino acid hydroxyproline, using preparative high-performance liquid chromatography (Prep-HPLC).

This method is more efficient in eliminating modern contamination in the bone collagen. P., suggesting the Vindija Neanderthals did not live more recently than others across Europe, and probably predate the arrival of anatomically modern humans in Eastern Europe.

This is an indicator of collagen preservation (20).

The results show that only one bone point sample (Vi-3446) had sufficient levels of nitrogen to warrant full sampling for collagen extraction and AMS dating; the remainder failed and therefore were not sampled further (, Table S1).

Radiocarbon dating of Neanderthal remains recovered from Vindija Cave (Croatia) initially revealed surprisingly recent results: 28,000–29,000 B. This implied the remains could represent a late-surviving, refugial Neanderthal population and suggested they could have been responsible for producing some of the early Upper Paleolithic artefacts more usually produced by anatomically modern humans.

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